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A value of p Because QS mutants accumulate more glutamate than the wild type, we hypothesized that QS mutants may experience a hypoosmotic condition resulting in water influx into the cell.
To test this hypothesis, we measured the elasticity of individual cells immobilized on a surface as indicated by Young’s moduli (or elastic moduli) using atomic force microscopy (AFM) in contact mode.
The letters (a,b and c) above each mean represent groupings of statistical significance based on an analysis of variance (ANOVA) and pairwise Student’s t-tests between strains or an ANOVA/Tukey’s correction for multiple comparisons.1b), suggesting that glutamate uptake is negatively controlled by QS in B. In addition to differential uptake rates of glutamate between the wild type and the QS mutants, we determined whether the expression of genes involved in nitrogen metabolism is controlled by QS because glutamate levels can be influenced by the glutamine synthetase (GS) and glutamine oxoglutarate aminotransferase (GOGAT) cycles.We compared the expression levels of the gln A (locus ID: bglu_1g25000), glt B (locus ID: bglu_1g03130), and gdh A (locus ID: bglu_1g05580) genes, which encode GS, GOGAT, and glutamate dehydrogenase (GDH), respectively, in the wild type, the tof I mutant BGS2, and the qsm R mutant BGS9.If your basket qualifies for a Buy Now, Pay Later plan, there’s nothing to pay for the length of the plan.Avoid any interest if you pay off the plan in full before it ends and keep your account up to date.